5-tRF-His, tRNA-derived fragments, regulate CKAP2 to inhibit the proliferation of breast cancer
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Author(s)
Abstract
tRNA derived fragments are differentiated expressed in human breast cancer( BC) tissues. Among of them, 5-tRF-His attracted our attention due to its potential role in breast cancer progression. PCR and in situ hybridization were used to measure the expression and location of 5-tRF-His. The role of 5-tRF-His in vitro was explored by cell proliferation assays and flow cytometery. The effect of 5-tRF-His in vivo was performed by Xenograft experiments and immunohistochemistry. We determined the derivation of 5-tRF-His by northern blot and clarified the mechanism of 5-tRF-His by immunoprecipitation and western blot. We observed that strong downregulation of 5-tRF-His in clinical BC samples. Reduced 5-tRF-His can lead to lymph node metastasis. 5-tRF-His, a tRNA-derived fragment linked to pan-ago, regulates the proliferation and apoptosis of BC cells and inhibits BC tumor growth explant tumor in vivo. The increase in proliferation and the decrease in apoptosis induced by the 5-tRF-His inhibitor were reversed by si-CKAP2. 5-tRF-His inhibits BC development in vivo and vitro. The 5-tRF-His/CKAP2/erk2 axis affects the proliferation and apoptosis of BC cells, imply that 5-tRF-His may be a promising tumor marker of BC patients serum and treatment target for BC therapy.
Keywords
breast cancer; serum; tRNA-derived fragments; CKAP2; proliferation; ERK2 signaling pathway
Cite this paper
Xun Tang, Pan Jiang, Huanhuan Chen, Jun Wu, Xiaodong Xie, Xuelian Mao, Dongping Mo, Li Tang, Feng Yan,
5-tRF-His, tRNA-derived fragments, regulate CKAP2 to inhibit the proliferation of breast cancer
, SCIREA Journal of Clinical Medicine.
Volume 5, Issue 2, April 2020 | PP. 8-29.
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