A dispersive solid phase extraction of hippuric acid in urine by silica nanocomposite supported onto silica gel prior to HPLC

Volume 2, Issue 2, April 2017     |     PP. 21-41      |     PDF (845 K)    |     Pub. Date: April 5, 2017
DOI:    348 Downloads     7612 Views  

Author(s)

Mohamad Raizul Zinalibdin, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia; Department of Chemistry, Johor Branch, Jalan Abdul Samad, 80100 Johor Bahru, Johor, Malaysia
Jafariah Jaafar, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
Zaiton Abdul Majid, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
Mohd Marsin Sanagi, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia

Abstract
In this study, a novel adsorbent material based on silica nanocomposite supported silica gel was successfully developed for extraction of hippuric acid. This adsorbent material was prepared via sol-gel method using 3-(propylmethacrylate)trimethoxysilane/silica nanocomposite (SN) acetic acid as catalyst, hippuric acid (HA) as template, acetonitrile as solvent and supported by silica gel. The morphology of SN was observed using field emission scanning electron microscopy (FESEM) while texture properties by N2 adsorption analysis. The silica nanocomposite was employed as a sorbent for extraction of hippuric acid. The effect of precursor amount, and template quantity on the sorption characteristic of SN was evaluated. Adsorption studies were conducted to identify the SN with the best sorption capacity for the extraction of hippuric acid. The adsorption carried out using 10 mL of sample or hippuric acid standard (200 mg/L), 0.2 g SN, 15 minutes stirring time and at pH 6. The HA was then desorbed into 10 mL methanol: acetic acid (9:1) solution by ultrasonication for 10 minutes. The SN prepared using 800 µL of 3-(propylmethacrylate)trimethoxysilane), (0.30 mmol/L of hippuric acid), 700µL acetonitrile as solvent and 600 µL of acetic acid was found to exhibit better sorption for hippuric acid. The results show percentage recovery around 97.9-99.6% and relative standard deviation at 0.4-4.6%, respectively. The silica nanocomposite supported with silica gel performance validation were analysed by high performance liquid chromatography (HPLC) at 225 nm of UV detector.

Keywords
Silica nanocomposite, hippuric acid, sol-gel, High performance liquid chromatography.

Cite this paper
Mohamad Raizul Zinalibdin, Jafariah Jaafar, Zaiton Abdul Majid, Mohd Marsin Sanagi, A dispersive solid phase extraction of hippuric acid in urine by silica nanocomposite supported onto silica gel prior to HPLC , SCIREA Journal of Chemistry. Volume 2, Issue 2, April 2017 | PP. 21-41.

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