Establishment of a Livin-gene silencing system and its effect on the sensitivity of Cal 27 with regard to 5-fluorouracil
DOI: 10.54647/cm32648 81 Downloads 3904 Views
Author(s)
Abstract
Objective: This study aims at downregulating the expression of Livin through shRNA and exploring the sensitivity of Cal 27 to 5-fluorouracil (5-FU), so as to provide some help in resolving the chemotherapy resistance of oral cancer.
Methods: The Livin-targeting shRNA sequence was designed to construct a lentivirus and infect Cal 27 to obtain a stable Livin-silencing cell strain. After being treated with 20 μM, 40 μM and 60 μM of 5-fluorouracil for 24 h, cell apoptosis and cell viability were measured by flow cytometry and MTT assay, respectively.
Results: A shRNA-based lentivirus targeting Livin was successfully designed and constructed. After the silencing of Livin, the sensitivity and cell apoptosis of Cal 27 to 5-FU were dramatically elevated, and cell viability was significantly reduced (P<0.05). Moreover, the inhibition of Caspase 3 was observed.
Conclusion: The expression level of Livin was downregulated in Cal 27 after shRNA transfection, which increased the sensitivity of Cal to 5-FU. The underlying mechanism is the blockage of the activation of Caspase 3. Therefore, Livin may serve as a promising target for oral cancer treatment.
Keywords
Mouth Neoplasms; Inhibitor of Apoptosis Proteins; Drug Therapy; Caspase 3; Fluorouracil; Gene Silencing
Cite this paper
Zixiao Huang, Ziwei Cui, Ruoshan Qin, Xuanxuan Yao, Hongli Zhou, Ru Guo, Xiaodong Qin, Xiangyi He,
Establishment of a Livin-gene silencing system and its effect on the sensitivity of Cal 27 with regard to 5-fluorouracil
, SCIREA Journal of Clinical Medicine.
Volume 6, Issue 6, December 2021 | PP. 474-487.
10.54647/cm32648
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